ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of hypobaric hypoxia pretreatment on surgically induced endometriosis in rats.</p><p><b>METHODS</b>Six rats were randomized into 2 groups and exposed to hypoxia (8% O) and normoxia (21% O) for 8 h. The uterine endometrium was intraperitoneally implanted into estrogen-treated ovariectomized Lewis rat, and the growth and quality of the implants were measured. The changes in apoptosis, protein and gene expressions in the serum, abdominis effusion fluids and implants were tested by ELISA, immunohistochemical staining, TUNNEL assay, Western blotting and RT-PCR.</p><p><b>RESULTS</b>The volume of the implants in the hypoxic pretreatment group was significantly increased compared with the normoxia group. High expressions of Ki67, CD31, VEGF, and HIF-1α and lowered cell apoptosis were found in the hypoxia-pretreated implants compared with the normoxic group. VEGF level in the serum and peritoneal fluid were increased in hypoxia-pretreated group, but TNFα level was comparable between the 2 groups.</p><p><b>CONCLUSION</b>Hypoxia play an important role in the occurrence and progression of endometriosis by increasing cell proliferation and angiogenesis and decreasing cell apoptosis in the implants in the rat model.</p>
Subject(s)
Animals , Female , Rats , Allografts , Apoptosis , Ascitic Fluid , Blotting, Western , Cell Proliferation , Endometriosis , Therapeutics , Endometrium , Transplantation , Hypoxia , Hypoxia-Inducible Factor 1, alpha Subunit , Metabolism , Ischemic Preconditioning , Neovascularization, Pathologic , Rats, Inbred Lew , Vascular Endothelial Growth Factor A , BloodABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of pituitary tumor transforming gene (PTTG) in human renal clear cell carcinoma (RCCC) and its significance.</p><p><b>METHODS</b>PTTG protein expression was detected by immunohistochemistry in 87 RCCC and 45 paired normal renal tissues.</p><p><b>RESULTS</b>PTTG was expressed differentially between the normal renal and RCCC tissues. Compared with normal tissues, the primary RCCC tissues showed significantly increased expression of PTTG protein (P<0.001). The positive rate of PTTG protein was positively correlated to the tumor size, clinical stage and Fuhrman grade of RCCC (P=0.009, 0.008 and 0.035, respectively).</p><p><b>CONCLUSION</b>Increased PTTG protein expression may be involved in the carcinogenesis and progression of RCCC.</p>